Professor Agnes Makhene holds a PhD in Nursing Education, MCur in Nursing Education, BCur (Ed et Admin), Diplomas in Community Nursing Science, Midwifery and General Nursing. She has 35 years of clinical experience gathered both in the public and private sector. Her teaching experience includes private, public colleges as well as university since 2006. Professor Agnes Makhene has presented research papers at several international conferences, has supervised to completion a number of Masters and PhD students, and published articles in accredited journals.

Professor Makhene has also served as an external examiner for Masters and PhD candidate nationally. She is an Associate Professor at the University of Johannesburg and a board member of the 16th Council of the SANC as the Deputy Chairperson of the Education Committee and Chairperson of the Professional Conduct Committee.